atcc crl 1721 1 pc12 strain Search Results


adherent pc 12 cells  (ATCC)
98
ATCC adherent pc 12 cells
Adherent Pc 12 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atcc+crl+1721+1+pc12+strain/PC-12/pm28992688-52-0-3
Average 98 stars, based on 1 article reviews
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pc12 adh  (ATCC)
95
ATCC pc12 adh
Fig. 5 (a) Effect of D-512 on 6-hydroxy- dopamine (6-OHDA)-induced nucleic condensation. Apoptotic nuclei were visualized via fluorescence dye 33342 staining. Apoptotic cells with high fluorescence intensity are indicated by arrows. Scale bar: 200 lm. (b) Effect of pre-treatment with varying concentration of D-512 followed by co-treatment with 75 lM 6-OHDA on DNA fragmentation in <t>PC12</t> cells. Lanes 1-4: marker, DNA laddering of control cells, DNA laddering in response to 75 lM 6-OHDA alone, or 10 lM D-512 alone. Lanes 5-7: DNA laddering in response to pre-treatment with varying concentrations of D-512 (10 lM, 5 lM and 1 lM) along with co-treatment with 75 lM 6-OHDA + 10 lM D-512, 75 lM 6-OHDA + 5 lM D-512, or 75 lM 6-OHDA + 1 lM D-512.
Pc12 Adh, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atcc+crl+1721+1+pc12+strain/PC-12+Adh/pm24848702-55-0-2
Average 95 stars, based on 1 article reviews
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adherent type  (ATCC)
94
ATCC adherent type
Fig. 5 (a) Effect of D-512 on 6-hydroxy- dopamine (6-OHDA)-induced nucleic condensation. Apoptotic nuclei were visualized via fluorescence dye 33342 staining. Apoptotic cells with high fluorescence intensity are indicated by arrows. Scale bar: 200 lm. (b) Effect of pre-treatment with varying concentration of D-512 followed by co-treatment with 75 lM 6-OHDA on DNA fragmentation in <t>PC12</t> cells. Lanes 1-4: marker, DNA laddering of control cells, DNA laddering in response to 75 lM 6-OHDA alone, or 10 lM D-512 alone. Lanes 5-7: DNA laddering in response to pre-treatment with varying concentrations of D-512 (10 lM, 5 lM and 1 lM) along with co-treatment with 75 lM 6-OHDA + 10 lM D-512, 75 lM 6-OHDA + 5 lM D-512, or 75 lM 6-OHDA + 1 lM D-512.
Adherent Type, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atcc+crl+1721+1+pc12+strain/PC-12+Adh%3B+Pheochromocytoma%3B+Rat/pmc10196224-65-5-7
Average 94 stars, based on 1 article reviews
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cell lines  (ATCC)
99
ATCC cell lines
Fig. 5 (a) Effect of D-512 on 6-hydroxy- dopamine (6-OHDA)-induced nucleic condensation. Apoptotic nuclei were visualized via fluorescence dye 33342 staining. Apoptotic cells with high fluorescence intensity are indicated by arrows. Scale bar: 200 lm. (b) Effect of pre-treatment with varying concentration of D-512 followed by co-treatment with 75 lM 6-OHDA on DNA fragmentation in <t>PC12</t> cells. Lanes 1-4: marker, DNA laddering of control cells, DNA laddering in response to 75 lM 6-OHDA alone, or 10 lM D-512 alone. Lanes 5-7: DNA laddering in response to pre-treatment with varying concentrations of D-512 (10 lM, 5 lM and 1 lM) along with co-treatment with 75 lM 6-OHDA + 10 lM D-512, 75 lM 6-OHDA + 5 lM D-512, or 75 lM 6-OHDA + 1 lM D-512.
Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atcc+crl+1721+1+pc12+strain/MCF7/10__1074_slash_jbc__m114__600288-55-3-24
Average 99 stars, based on 1 article reviews
cell lines - by Bioz Stars, 2026-10
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sh sy5y  (ATCC)
99
ATCC sh sy5y
Fig. 5 (a) Effect of D-512 on 6-hydroxy- dopamine (6-OHDA)-induced nucleic condensation. Apoptotic nuclei were visualized via fluorescence dye 33342 staining. Apoptotic cells with high fluorescence intensity are indicated by arrows. Scale bar: 200 lm. (b) Effect of pre-treatment with varying concentration of D-512 followed by co-treatment with 75 lM 6-OHDA on DNA fragmentation in <t>PC12</t> cells. Lanes 1-4: marker, DNA laddering of control cells, DNA laddering in response to 75 lM 6-OHDA alone, or 10 lM D-512 alone. Lanes 5-7: DNA laddering in response to pre-treatment with varying concentrations of D-512 (10 lM, 5 lM and 1 lM) along with co-treatment with 75 lM 6-OHDA + 10 lM D-512, 75 lM 6-OHDA + 5 lM D-512, or 75 lM 6-OHDA + 1 lM D-512.
Sh Sy5y, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atcc+crl+1721+1+pc12+strain/SH-SY5Y/bio_rxiv__852715-156-4-5
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pc12 cells  (LGC Standards)
92
LGC Standards pc12 cells
Functional analysis of Rgs4 promoter activity in transfected cells. Rgs4 constructs cloned into pGL4.11 were transfected into <t>PC12</t> cells. Levels of expression were determined by luciferase ( Luc ) assays and corrected against a co-transfected Renilla luciferase construct. A , expression levels (luciferase activity) of the different constructs relative to empty pGL4.11 (mean ± S.E., n = 8; *, p < 0.05 versus all other groups, ANOVA and post hoc test). The position of the −415 AP-1F, −155 AP-1R, and −42 TATA elements (see text) are indicated by filled symbols and are shown as open symbols where mutated ( Rgs4 -167m and Rgs4 -426m constructs, see text). B , Western blot of whole cell extracts (pineal 24.00 h, 50 μg; PC12 cells, 10 μg) probed sequentially with antisera to FRA-2 and GAPDH. Note the relatively high level of FRA-2 in unstimulated PC12 cells. Arrows indicate two (differentially phosphorylated ) FRA-2 bands. Horizontal bars indicate molecular weight markers.
Pc12 Cells, supplied by LGC Standards, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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Image Search Results


Fig. 5 (a) Effect of D-512 on 6-hydroxy- dopamine (6-OHDA)-induced nucleic condensation. Apoptotic nuclei were visualized via fluorescence dye 33342 staining. Apoptotic cells with high fluorescence intensity are indicated by arrows. Scale bar: 200 lm. (b) Effect of pre-treatment with varying concentration of D-512 followed by co-treatment with 75 lM 6-OHDA on DNA fragmentation in PC12 cells. Lanes 1-4: marker, DNA laddering of control cells, DNA laddering in response to 75 lM 6-OHDA alone, or 10 lM D-512 alone. Lanes 5-7: DNA laddering in response to pre-treatment with varying concentrations of D-512 (10 lM, 5 lM and 1 lM) along with co-treatment with 75 lM 6-OHDA + 10 lM D-512, 75 lM 6-OHDA + 5 lM D-512, or 75 lM 6-OHDA + 1 lM D-512.

Journal: Journal of neurochemistry

Article Title: The high-affinity D2/D3 agonist D512 protects PC12 cells from 6-OHDA-induced apoptotic cell death and rescues dopaminergic neurons in the MPTP mouse model of Parkinson's disease.

doi: 10.1111/jnc.12767

Figure Lengend Snippet: Fig. 5 (a) Effect of D-512 on 6-hydroxy- dopamine (6-OHDA)-induced nucleic condensation. Apoptotic nuclei were visualized via fluorescence dye 33342 staining. Apoptotic cells with high fluorescence intensity are indicated by arrows. Scale bar: 200 lm. (b) Effect of pre-treatment with varying concentration of D-512 followed by co-treatment with 75 lM 6-OHDA on DNA fragmentation in PC12 cells. Lanes 1-4: marker, DNA laddering of control cells, DNA laddering in response to 75 lM 6-OHDA alone, or 10 lM D-512 alone. Lanes 5-7: DNA laddering in response to pre-treatment with varying concentrations of D-512 (10 lM, 5 lM and 1 lM) along with co-treatment with 75 lM 6-OHDA + 10 lM D-512, 75 lM 6-OHDA + 5 lM D-512, or 75 lM 6-OHDA + 1 lM D-512.

Article Snippet: PC12 Adh (ATCC CRL1721.1TM, Manassas, VA, USA) cells, a rat adrenal pheochromocytoma cell line, were purchased from ATCC.

Techniques: Staining, Concentration Assay, Marker, DNA Laddering, Control

Functional analysis of Rgs4 promoter activity in transfected cells. Rgs4 constructs cloned into pGL4.11 were transfected into PC12 cells. Levels of expression were determined by luciferase ( Luc ) assays and corrected against a co-transfected Renilla luciferase construct. A , expression levels (luciferase activity) of the different constructs relative to empty pGL4.11 (mean ± S.E., n = 8; *, p < 0.05 versus all other groups, ANOVA and post hoc test). The position of the −415 AP-1F, −155 AP-1R, and −42 TATA elements (see text) are indicated by filled symbols and are shown as open symbols where mutated ( Rgs4 -167m and Rgs4 -426m constructs, see text). B , Western blot of whole cell extracts (pineal 24.00 h, 50 μg; PC12 cells, 10 μg) probed sequentially with antisera to FRA-2 and GAPDH. Note the relatively high level of FRA-2 in unstimulated PC12 cells. Arrows indicate two (differentially phosphorylated ) FRA-2 bands. Horizontal bars indicate molecular weight markers.

Journal: The Journal of Biological Chemistry

Article Title: Selective Genomic Targeting by FRA-2/FOSL2 Transcription Factor

doi: 10.1074/jbc.M110.201996

Figure Lengend Snippet: Functional analysis of Rgs4 promoter activity in transfected cells. Rgs4 constructs cloned into pGL4.11 were transfected into PC12 cells. Levels of expression were determined by luciferase ( Luc ) assays and corrected against a co-transfected Renilla luciferase construct. A , expression levels (luciferase activity) of the different constructs relative to empty pGL4.11 (mean ± S.E., n = 8; *, p < 0.05 versus all other groups, ANOVA and post hoc test). The position of the −415 AP-1F, −155 AP-1R, and −42 TATA elements (see text) are indicated by filled symbols and are shown as open symbols where mutated ( Rgs4 -167m and Rgs4 -426m constructs, see text). B , Western blot of whole cell extracts (pineal 24.00 h, 50 μg; PC12 cells, 10 μg) probed sequentially with antisera to FRA-2 and GAPDH. Note the relatively high level of FRA-2 in unstimulated PC12 cells. Arrows indicate two (differentially phosphorylated ) FRA-2 bands. Horizontal bars indicate molecular weight markers.

Article Snippet: PC12 cells (CRL-1721.1; LGC Standards, Teddington, Middlesex, UK) were grown in DMEM (Invitrogen) with 10% horse serum, 5% FBS (Invitrogen) and 1× antibiotic/antimycotic (Invitrogen) at 37 °C and 5% CO 2 .

Techniques: Functional Assay, Activity Assay, Transfection, Construct, Clone Assay, Expressing, Luciferase, Western Blot, Molecular Weight